Protein Synthesis 3: RNA Polymerase

Transcription과 Translation과정을 자세히 알아보자.

1. Transcription에 사용되는 Enzyme: RNA Polymerase

• Transcription is the process by which the code contained in the DNA molecule is transcribed into a mRNA molecule.
• Under the control of the cell’s metabolic processes.
• The gene must be activated before this process can begin.

2. The role of RNA Polymerase

1. Enzyme that directly controls the process
2. Moves 3’–>5’ direction
3. Makes a strand of mRNA using the single strand of DNA (template strand) –Only transcribe a gene length of DNA at a times.
4. Recognizes start and stop codes at the beginning and end of the gene.
5. Only enzyme involved in mRNA synthesis.
6. Unwinds the DNA

3. Terms

• Template strand: contains the information for the construction of a protein
• Coding strand: DNA has nucleotide sequence complementary to the template strand.
• mRNA: Complementary to the template strand. Containing same ‘message’ as the coding strand.

4. DNA처럼 RNA도 5′–>3’방향으로 자란다. (3′–>5′ 읽어나가기 때문에..)

• RNA grows in 5’–>3’ direction by adding new nucleotides to the 3’ end.
• Two polynucleotide strands are anti-parallel.