{"id":891,"date":"2010-05-13T01:01:54","date_gmt":"2010-05-12T12:01:54","guid":{"rendered":"http:\/\/science4all.blogtown.co.nz\/?p=891"},"modified":"2010-05-13T01:03:10","modified_gmt":"2010-05-12T12:03:10","slug":"biotechnology-recombinant-dna","status":"publish","type":"post","link":"https:\/\/science4all.blogtown.co.nz\/?p=891","title":{"rendered":"Biotechnology: Recombinant DNA"},"content":{"rendered":"<p><span style=\"color: #666699\"><strong>Question<\/strong><\/span><\/p>\n<p><span style=\"color: #666699\">Discuss the reasons why it is often impossible or more difficult to use bacteria as transgenic organisms to make many human proteins. <\/span><\/p>\n<p><strong>Answer<\/strong><\/p>\n<p>Many human genes are very large, with many intron regions in the gene. Bacterial DNA does not contain introns and amount of DNA which can be inserted is limited.<\/p>\n<p>Introns would have to be removed from human gene to make it small enough to fit into the plasmid. Protein required may need further processing beyond assembly of amino acids. Bacterial cells do not have\u00a0machinery\u00a0to carry out secondary and tertiary processes in creating a complex protein.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Question Discuss the reasons why it is often impossible or more difficult to use bacteria as transgenic organisms to make many human proteins. Answer Many human genes are very large, with many intron regions in the gene. Bacterial DNA does not contain introns and amount of DNA which can be inserted is limited. Introns would &hellip; <\/p>\n<p class=\"link-more\"><a href=\"https:\/\/science4all.blogtown.co.nz\/?p=891\" class=\"more-link\">Continue reading<span class=\"screen-reader-text\"> &#8220;Biotechnology: Recombinant DNA&#8221;<\/span><\/a><\/p>\n","protected":false},"author":1509,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"slim_seo":{"title":"Biotechnology: Recombinant DNA - science4all","description":"Question Discuss the reasons why it is often impossible or more difficult to use bacteria as transgenic organisms to make many human proteins. Answer Many human"},"footnotes":""},"categories":[1],"tags":[],"class_list":["post-891","post","type-post","status-publish","format-standard","hentry","category-uncategorized"],"_links":{"self":[{"href":"https:\/\/science4all.blogtown.co.nz\/index.php?rest_route=\/wp\/v2\/posts\/891","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/science4all.blogtown.co.nz\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/science4all.blogtown.co.nz\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/science4all.blogtown.co.nz\/index.php?rest_route=\/wp\/v2\/users\/1509"}],"replies":[{"embeddable":true,"href":"https:\/\/science4all.blogtown.co.nz\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=891"}],"version-history":[{"count":3,"href":"https:\/\/science4all.blogtown.co.nz\/index.php?rest_route=\/wp\/v2\/posts\/891\/revisions"}],"predecessor-version":[{"id":893,"href":"https:\/\/science4all.blogtown.co.nz\/index.php?rest_route=\/wp\/v2\/posts\/891\/revisions\/893"}],"wp:attachment":[{"href":"https:\/\/science4all.blogtown.co.nz\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=891"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/science4all.blogtown.co.nz\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=891"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/science4all.blogtown.co.nz\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=891"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}