{"id":873,"date":"2010-05-12T23:56:27","date_gmt":"2010-05-12T10:56:27","guid":{"rendered":"http:\/\/science4all.blogtown.co.nz\/?p=873"},"modified":"2010-05-13T01:14:39","modified_gmt":"2010-05-12T12:14:39","slug":"biotechnology-exam-q-and-a","status":"publish","type":"post","link":"https:\/\/science4all.blogtown.co.nz\/?p=873","title":{"rendered":"Biotechnology: Wollemi pines"},"content":{"rendered":"<p><span style=\"color: #666699\"><strong>Question one<\/strong><\/span><\/p>\n<p><span style=\"color: #666699\">The <\/span><em><span style=\"color: #666699\">Wollemi <\/span><\/em><span style=\"color: #666699\"><span style=\"color: #666699\">pin<\/span>es have been shown to be susceptible to the die back fungus(<\/span><em><span style=\"color: #666699\">Phytophythora cinnamomi<\/span><\/em><span style=\"color: #666699\">) that attacks trees in other Australian native forests. One species of pine has resistance to this fungus because of a single gene. Discuss how biotechnological technique could be used to develop Wollemi pines that are resistant to the die-back fungus. In your discussion, include that may result from the\u00a0procedures\u00a0involved.<\/span><\/p>\n<p><span style=\"color: #993300\">Answer<\/span><\/p>\n<p><span style=\"color: #993300\"><em><span style=\"color: #000000\">Wollemi <\/span><\/em><span style=\"color: #000000\">pines could be made resistant to die-back fungus using process of transgenesis \u00a0&#8211; taking organism resistant to fungus, identify resistant gene, cut using restricti<\/span><\/span><\/p>\n<p><span style=\"color: #993300\"><span style=\"color: #000000\">on enzymes, cut plasmid to infect pine.<\/span><\/span><\/p>\n<p><span style=\"color: #993300\"><span style=\"color: #000000\">Concerns <\/span><\/span><\/p>\n<p><span style=\"color: #993300\"><span style=\"color: #000000\">Gene product may interact with products of another gene, transfer process may disrupt ability for gene to offer\u00a0resistance, gene may insert in middle of a vital <\/span><em><span style=\"color: #000000\">Wollemi <\/span><\/em><span style=\"color: #000000\">pine gene and disrupt ability of that gene to function (produce non functional protein), gene may only function sporadically because not inserted into a chromosome therefore disrupting its ability to offer resistance.<\/span><\/span><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Question one The Wollemi pines have been shown to be susceptible to the die back fungus(Phytophythora cinnamomi) that attacks trees in other Australian native forests. One species of pine has resistance to this fungus because of a single gene. Discuss how biotechnological technique could be used to develop Wollemi pines that are resistant to the &hellip; <\/p>\n<p class=\"link-more\"><a href=\"https:\/\/science4all.blogtown.co.nz\/?p=873\" class=\"more-link\">Continue reading<span class=\"screen-reader-text\"> &#8220;Biotechnology: Wollemi pines&#8221;<\/span><\/a><\/p>\n","protected":false},"author":1509,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"slim_seo":{"title":"Biotechnology: Wollemi pines - science4all","description":"Question one The Wollemi pin es have been shown to be susceptible to the die back fungus( Phytophythora cinnamomi ) that attacks trees in other Australian nativ"},"footnotes":""},"categories":[1],"tags":[],"class_list":["post-873","post","type-post","status-publish","format-standard","hentry","category-uncategorized"],"_links":{"self":[{"href":"https:\/\/science4all.blogtown.co.nz\/index.php?rest_route=\/wp\/v2\/posts\/873","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/science4all.blogtown.co.nz\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/science4all.blogtown.co.nz\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/science4all.blogtown.co.nz\/index.php?rest_route=\/wp\/v2\/users\/1509"}],"replies":[{"embeddable":true,"href":"https:\/\/science4all.blogtown.co.nz\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=873"}],"version-history":[{"count":2,"href":"https:\/\/science4all.blogtown.co.nz\/index.php?rest_route=\/wp\/v2\/posts\/873\/revisions"}],"predecessor-version":[{"id":900,"href":"https:\/\/science4all.blogtown.co.nz\/index.php?rest_route=\/wp\/v2\/posts\/873\/revisions\/900"}],"wp:attachment":[{"href":"https:\/\/science4all.blogtown.co.nz\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=873"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/science4all.blogtown.co.nz\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=873"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/science4all.blogtown.co.nz\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=873"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}