{"id":33,"date":"2009-12-06T22:34:40","date_gmt":"2009-12-06T09:34:40","guid":{"rendered":"http:\/\/science4all.blogtown.co.nz\/?p=33"},"modified":"2009-12-07T11:45:04","modified_gmt":"2009-12-06T22:45:04","slug":"dna-replication-process-steps-2","status":"publish","type":"post","link":"https:\/\/science4all.blogtown.co.nz\/?p=33","title":{"rendered":"DNA Replication Process Steps (2)"},"content":{"rendered":"<ul>\n<li><strong>Helicase: <\/strong>The enzyme splits and unwinds the 2-strnaded DNA molecules.<\/li>\n<li><strong>DNA Polymerase\u00a0III<\/strong> : Extends RNA primer with short length of complementary DNA.<\/li>\n<li><strong>RNA Polymerase: <\/strong>Synthesizes a short RNA primer which is later removed.<\/li>\n<li><strong>DNA Polymerase\u00a0I: <\/strong>Digests RNA primer and replaces it with DNA.<\/li>\n<li><strong>DNA Ligase: <\/strong>Joins neighbouring fragments together into longer strands.<\/li>\n<\/ul>\n<p style=\"text-align: center\">&#8230;&#8230;&#8230;&#8230;&#8230;&#8230;&#8230;&#8230;&#8230;&#8230;&#8230;&#8230;&#8230;&#8230;&#8230;&#8230;&#8230;&#8230;&#8230;..<\/p>\n<p><strong>Leading strand<\/strong><\/p>\n<p>\u2022Leading strand is the new strand that grows continuously toward the replication fork.<\/p>\n<p>\u2022\u00a0The new strand is 5\u2019&#8211;&gt;3\u2019 direction. It is also complementary to 3\u2019&#8211;&gt;5\u2019 parent strand.<\/p>\n<p>\u2022DNA polymerase reads the parent strand in 3\u2019 to 5\u2019 direction, while building the new strand in opposite (5\u2019&#8211;&gt;3\u2019) direction.<\/p>\n<p style=\"text-align: center\">&#8230;&#8230;&#8230;&#8230;&#8230;&#8230;&#8230;&#8230;&#8230;&#8230;&#8230;&#8230;&#8230;&#8230;&#8230;&#8230;&#8230;&#8230;&#8230;..<\/p>\n<p><strong>Lagging strand<\/strong><\/p>\n<p>Okazaki Fragment: The lagging strand is formed in fragments, between 1000 and 2000 nucleotides long \u2013 called Okazaki fragments.<\/p>\n<p>\u2022Since new nucleotides can only be added at the 3\u2019end, each new half-strand is synthesized in the 5\u2019&#8211;&gt;3\u2019 direction.<\/p>\n<p>\u2022At the 5\u2019end of the old strand, the new strand is synthesized in separate bits called Okazaki fragments. These grow addition at their 3\u2019ends and are then joined up by DNA ligase.<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" src=\"http:\/\/faculty.irsc.edu\/FACULTY\/TFischer\/images\/DNA%20replication.jpg\" alt=\"\" width=\"479\" height=\"254\" \/><\/p>\n<p style=\"text-align: center\">&#8230;&#8230;&#8230;&#8230;&#8230;&#8230;&#8230;&#8230;&#8230;&#8230;&#8230;&#8230;&#8230;&#8230;&#8230;&#8230;&#8230;&#8230;&#8230;..<\/p>\n<p>You should be able to explain the steps involved in DNA Replication\u00a0&amp;\u00a0role of the enzymes in DNA replication.<\/p>\n<p>To help you with the understanding of this concept watch this Youtube video: <a title=\"DNA replication process and Okazaki fragment\" href=\"http:\/\/www.youtube.com\/watch?v=teV62zrm2P0\" target=\"_blank\">http:\/\/www.youtube.com\/watch?v=teV62zrm2P0<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Helicase: The enzyme splits and unwinds the 2-strnaded DNA molecules. DNA Polymerase\u00a0III : Extends RNA primer with short length of complementary DNA. RNA Polymerase: Synthesizes a short RNA primer which is later removed. DNA Polymerase\u00a0I: Digests RNA primer and replaces it with DNA. DNA Ligase: Joins neighbouring fragments together into longer strands. &#8230;&#8230;&#8230;&#8230;&#8230;&#8230;&#8230;&#8230;&#8230;&#8230;&#8230;&#8230;&#8230;&#8230;&#8230;&#8230;&#8230;&#8230;&#8230;.. Leading strand &hellip; <\/p>\n<p class=\"link-more\"><a href=\"https:\/\/science4all.blogtown.co.nz\/?p=33\" class=\"more-link\">Continue reading<span class=\"screen-reader-text\"> &#8220;DNA Replication Process Steps (2)&#8221;<\/span><\/a><\/p>\n","protected":false},"author":1509,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"slim_seo":{"title":"DNA Replication Process Steps (2) - science4all","description":"Helicase: The enzyme splits and unwinds the 2-strnaded DNA molecules. DNA Polymerase\u00a0III : Extends RNA primer with short length of complementary DNA. RNA Polyme"},"footnotes":""},"categories":[1],"tags":[],"class_list":["post-33","post","type-post","status-publish","format-standard","hentry","category-uncategorized"],"_links":{"self":[{"href":"https:\/\/science4all.blogtown.co.nz\/index.php?rest_route=\/wp\/v2\/posts\/33","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/science4all.blogtown.co.nz\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/science4all.blogtown.co.nz\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/science4all.blogtown.co.nz\/index.php?rest_route=\/wp\/v2\/users\/1509"}],"replies":[{"embeddable":true,"href":"https:\/\/science4all.blogtown.co.nz\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=33"}],"version-history":[{"count":10,"href":"https:\/\/science4all.blogtown.co.nz\/index.php?rest_route=\/wp\/v2\/posts\/33\/revisions"}],"predecessor-version":[{"id":46,"href":"https:\/\/science4all.blogtown.co.nz\/index.php?rest_route=\/wp\/v2\/posts\/33\/revisions\/46"}],"wp:attachment":[{"href":"https:\/\/science4all.blogtown.co.nz\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=33"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/science4all.blogtown.co.nz\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=33"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/science4all.blogtown.co.nz\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=33"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}